Conventional cytology lacks precision due to one or more of the following reasons,
To overcome these problems Liquid-Based Cytology Technique is a necessary welcome. It is being adopted in many countries for Gynae/Non-Gynae samples. Here, for example in Pap smear, instead of making smears on slide the spatula is immersed in Liquid(preservative-an alcoholic content) and sent to the laboratory. The liquid is processed for removing obscuring material, mucus and blood; with clearing solution and centrifuged by conventional centrifugation for collecting cells. Supernatant is discarded and pellicle so formed is admixed with cellular base solution which is finally transferred to clean slide by pasteur pipette. Slides are dried at 40 degree (incubator) and stained by conventional techniques having monolayered cellularity.
The cost has been considerably reduced with newer manufacturers supplying kits at reduced price and omitting with automated technology adopted in its initial years. We see bright future of liquid based cytology helping in diagnosing lethal diseases early and with confidence.